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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DKK3 Monoclonal antibody (66758-1-Ig)

    WB analysis using 66758-1-Ig (same clone as 66758-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66758-1-Ig (DKK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using DKK3 Monoclonal antibody (66758-1-Ig)

    IHC staining of human heart using 66758-1-Ig (same clone as 66758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using DKK3 Monoclonal antibody (66758-1-Ig)

    IHC staining of human heart using 66758-1-Ig (same clone as 66758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DKK3 Monoclonal antibody (66758-1-Ig)

    IHC staining of mouse brain using 66758-1-Ig (same clone as 66758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DKK3 Monoclonal antibody (66758-1-Ig)

    IHC staining of mouse brain using 66758-1-Ig (same clone as 66758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DKK3 Monoclonal antibody (66758-1-Ig)

    IF Staining of HEK-293 using 66758-1-Ig (same clone as 66758-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DKK3 antibody (66758-1-Ig, Clone: 4E6H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

DKK3 Monoclonal antibody, PBS Only

DKK3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66758-1-PBS
Clone No.4E6H6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Dickkopf 3, Dickkopf related protein 3, Dkk 3, DKK3, hDkk 3, REIC, RIG

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66758-1-PBS targets DKK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag23615

Product name: Recombinant human DKK3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 37-350 aa of BC007660

Sequence: ALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALGEPAAAAAALLGGEEI

相同性解析による交差性が予測される生物種
Full Name dickkopf homolog 3 (Xenopus laevis)
Calculated molecular weight 38 kDa
Observed molecular weight 40 kDa, 50-55 kDa
GenBank accession numberBC007660
Gene Symbol DKK3
Gene ID (NCBI) 27122
RRIDAB_2882104
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UBP4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.

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