Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, mouse brain tissue, K-562 cells, HeLa cells, mouse heart tissue, rat brain tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 59 publications below |
| IHC | See 22 publications below |
| IF | See 5 publications below |
Product Information
21112-1-AP targets DKK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15110 Product name: Recombinant human DKK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-191 aa of BC001539 Sequence: TLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLR 相同性解析による交差性が予測される生物種 |
| Full Name | dickkopf homolog 1 (Xenopus laevis) |
| Calculated molecular weight | 266 aa, 29 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC001539 |
| Gene Symbol | DKK1 |
| Gene ID (NCBI) | 22943 |
| RRID | AB_10733097 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DKK1 antibody 21112-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Gut microbial alterations in arginine metabolism determine bone mechanical adaptation | ||
Nat Commun Cancer stem cell regulated phenotypic plasticity protects metastasized cancer cells from ferroptosis.
| ||
Nat Commun Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics | ||
Nat Commun Stroma-derived Dickkopf-1 contributes to the suppression of NK cell cytotoxicity in breast cancer | ||
Theranostics BPI-9016M, a c-Met inhibitor, suppresses tumor cell growth, migration and invasion of lung adenocarcinoma via miR203-DKK1.
| ||
J Exp Clin Cancer Res Depleting PTOV1 sensitizes non-small cell lung cancer cells to chemotherapy through attenuating cancer stem cell traits. |