Validation Data Gallery
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Tested Applications
| Positive WB detected in | U2OS cells, HSC-T6 cells, LNCaP cells, Hela cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
67623-1-Ig targets DIS3L2 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29992 Product name: Recombinant human DIS3L2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-170 aa of BC030113 Sequence: AGALGYRERLDMAPDTLQKQADHCNDSRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQKRIYCNALALRSHHFQKVGKKPELTLVWEPEDMEQEPAQQVITIFSLVEVVLQAEYTALKYSAILKRPGTQGHLGPEKEEEESDGEPEDSSTS 相同性解析による交差性が予測される生物種 |
| Full Name | DIS3 mitotic control homolog (S. cerevisiae)-like 2 |
| Calculated molecular weight | 885 aa, 99 kDa |
| Observed molecular weight | 65 kDa, 99 kda |
| GenBank accession number | BC030113 |
| Gene Symbol | DIS3L2 |
| Gene ID (NCBI) | 129563 |
| RRID | AB_2882825 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8IYB7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for DIS3L2 antibody 67623-1-Ig | Download protocol |
| WB protocol for DIS3L2 antibody 67623-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential | ||
Cell Rep Cytoplasmic DIS3 is an exosome-independent endoribonuclease with catalytic activity toward circular RNAs |
