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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DIS3 Polyclonal antibody (29664-1-AP)

    WB analysis using 29664-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29664-1-AP (DIS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DIS3 Polyclonal antibody (29664-1-AP)

    IHC staining of human colon cancer using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DIS3 Polyclonal antibody (29664-1-AP)

    IHC staining of mouse testis using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DIS3 Polyclonal antibody (29664-1-AP)

    IHC staining of mouse testis using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DIS3 Polyclonal antibody (29664-1-AP)

    IHC staining of mouse testis using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DIS3 Polyclonal antibody (29664-1-AP)

    IHC staining of human colon cancer using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DIS3 Polyclonal antibody (29664-1-AP)

    IHC staining of human colon cancer using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DIS3 Polyclonal antibody (29664-1-AP)

    IHC staining of human ovary tumor using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DIS3 Polyclonal antibody (29664-1-AP)

    IHC staining of human ovary tumor using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DIS3 Polyclonal antibody (29664-1-AP)

    IF Staining of HeLa using 29664-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIS3 antibody (29664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

DIS3 Polyclonal antibody

DIS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29664-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DIS 3, Dis-3, dis3p, EC:3.1.13.-, EXOSC11

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29664-1-AP targets DIS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31711

Product name: Recombinant human DIS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 607-958 aa of NM_014953

Sequence: TTSLRGLNKLAKILKKRRIEKGALTLSSPEVRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTDTAKSLAESLDQAESPTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIGADCTYPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKLGK

相同性解析による交差性が予測される生物種
Full Name DIS3 mitotic control homolog (S. cerevisiae)
Calculated molecular weight109KD
Observed molecular weight110 kDa
GenBank accession numberNM_014953
Gene Symbol DIS3
Gene ID (NCBI) 22894
RRIDAB_2918336
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2L1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.

Protocols

Product Specific Protocols
IF protocol for DIS3 antibody 29664-1-APDownload protocol
IHC protocol for DIS3 antibody 29664-1-APDownload protocol
WB protocol for DIS3 antibody 29664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

PABPC1 mediates degradation of C9orf72-FTLD/ALS GGGGCC repeat RNA

Authors - Ryota Uozumi
View Article
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