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Validation Data Gallery

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  • Western Blot (WB) analysis of T-47D cells using DIO2 Monoclonal antibody (66813-1-Ig)

    WB analysis of T-47D using 66813-1-Ig (same clone as 66813-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using DIO2 Monoclonal antibody (66813-1-Ig)

    WB analysis of MCF-7 using 66813-1-Ig (same clone as 66813-1-PBS)

    WB result of DIO2 antibody (66813-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIO2 transfected MCF-7 cells. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using DIO2 Monoclonal antibody (66813-1-Ig)

    WB analysis of LNCaP using 66813-1-Ig (same clone as 66813-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DIO2 Monoclonal antibody (66813-1-Ig)

    IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DIO2 Monoclonal antibody (66813-1-Ig)

    IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DIO2 Monoclonal antibody (66813-1-Ig)

    IHC staining of mouse skeletal muscle using 66813-1-Ig (same clone as 66813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DIO2 Monoclonal antibody (66813-1-Ig)

    IHC staining of mouse skeletal muscle using 66813-1-Ig (same clone as 66813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DIO2 Monoclonal antibody, PBS Only

DIO2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66813-1-PBS
Clone No.1F2A1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

5DII, D2, DIOII, EC:1.21.99.4, ITDI2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66813-1-PBS targets DIO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24057

Product name: Recombinant human DIO2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 171-273 aa of BC074882

Sequence: AIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEKNFSKRUKKTRLAG

相同性解析による交差性が予測される生物種
Full Name deiodinase, iodothyronine, type II
Calculated molecular weight 273 aa, 31 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC074882
Gene Symbol DIO2
Gene ID (NCBI) 1734
RRIDAB_2882156
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ92813
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.

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