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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DICER1 Polyclonal antibody (29984-1-AP)

    WB analysis using 29984-1-AP (same clone as 29984-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29984-1-AP (DICER1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29984-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using DICER1 Polyclonal antibody (29984-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 29984-1-AP (same clone as 29984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29984-1-AP (DICER1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29984-1-PBS in a different storage buffer formulation.

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DICER1 Polyclonal antibody, PBS Only

DICER1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29984-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

HERNA, Helicase MOI, Endoribonuclease Dicer, EC:3.1.26.3, DICER

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29984-1-PBS targets DICER1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DICER1 fusion protein Ag30368 相同性解析による交差性が予測される生物種
Full Name dicer 1, ribonuclease type III
Calculated molecular weight 219 kDa
Observed molecular weight90 kDa, 220-250 kDa
GenBank accession numberNM_030621
Gene Symbol DICER1
Gene ID (NCBI) 23405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPY3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DICER1 functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. DICER1 cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, and producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation.