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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    WB result of DIAPH3 antibody (14342-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of HEK-293 using 14342-1-AP (same clone as 14342-1-PBS)

    WB result of DIAPH3 antibody (14342-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of DU 145 using 14342-1-AP (same clone as 14342-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of HEK-293 using 14342-1-AP (same clone as 14342-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    IP experiment of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    IP result of anti-DIAPH3 (IP:14342-1-AP, 4ug; Detection:14342-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of human cervical cancer using 14342-1-AP (same clone as 14342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of mouse testis using 14342-1-AP (same clone as 14342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of rat testis using 14342-1-AP (same clone as 14342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of human prostate cancer using 14342-1-AP (same clone as 14342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of human prostate cancer using 14342-1-AP (same clone as 14342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of human cervical cancer using 14342-1-AP (same clone as 14342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    IF Staining of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody (14342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    IF Staining of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    Immunofluorescent analysis of HeLa cells using 14342-1-AP (DIAPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DIAPH3 Polyclonal antibody, PBS Only

DIAPH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14342-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14342-1-PBS targets DIAPH3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5656

Product name: Recombinant human DIAPH3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 500-850 aa of BC048963

Sequence: DQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEKYPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKVCNHGNKPYL

相同性解析による交差性が予測される生物種
Full Name diaphanous homolog 3 (Drosophila)
Calculated molecular weight 137 kDa
Observed molecular weight 125-137 kDa
GenBank accession numberBC048963
Gene Symbol DIAPH3
Gene ID (NCBI) 81624
RRIDAB_2092930
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSV4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

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