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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DIABLO Monoclonal antibody (68480-1-Ig)

    WB analysis using 68480-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using DIABLO Monoclonal antibody (68480-1-Ig)

    WB analysis using 68480-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Smac/DIABLO Monoclonal antibody (68480-1-Ig)

    IF Staining of HepG2 using 68480-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Smac/DIABLO antibody (68480-1-Ig, Clone: 2F12C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

Smac/DIABLO Monoclonal antibody

Smac/DIABLO Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68480-1-Ig
Clone No.2F12C9

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

DIABLO, 2F12C9, DFNA64, Diablo homolog, mitochondrial, Diablo IAP-binding mitochondrial protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, K-562 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68480-1-Ig targets Smac/DIABLO in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag32774

Product name: Recombinant human DIABLO protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-239 aa of BC011909

Sequence: MAALKSWLSRSVTSFFRYRQCLCVPVVANFKKRCFSELIRPWHKTVTIGFGVTLCAVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED

相同性解析による交差性が予測される生物種
Full Name diablo homolog (Drosophila)
Calculated molecular weight 27 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC011909
Gene Symbol DIABLO
Gene ID (NCBI) 56616
RRIDAB_3085191
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9NR28
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)

Protocols

Product Specific Protocols
WB protocol for Smac/DIABLO antibody 68480-1-IgDownload protocol
IF protocol for Smac/DIABLO antibody 68480-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols