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Species Reactivity

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  • Featured Product
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DHX37 Polyclonal antibody

DHX37 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29741-1-AP

Synonyms

DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517

Validation Data Gallery

Applications:
  • WB analysis using 29741-1-AP

    WB analysis using 29741-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29741-1-AP (DHX37 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 29741-1-AP

    WB analysis of HEK-293 using 29741-1-AP

    WB result of DHX37 antibody (29741-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX37 transfected HEK-293 cells.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

29741-1-AP targets DHX37 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX37 fusion protein Ag29909 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 37
Calculated molecular weight 1157 aa, 130 kDa
Observed molecular weight130 kDa
GenBank accession numberBC037964
Gene symbol DHX37
Gene ID (NCBI) 57647
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHX37, also known as DDX37, KIAA1517. It is an ATP-bound RNA helicase that plays a role in the maturation of small ribosomal subunits in ribosomal biogenesis (PMID:30582406). The presence of DHX37 in early pre-ribosomal particles was monitored by a quality control pathway, and failure to recruit DHX37 resulted in pre-RRNA degradation. DHX37 binds directly to U3 small nucleolar RNA (snoRNA), and the catalytic activity of the helicase is required for dissociation of U3 small nucleolar RNA from the pre-ribosomal complex.In addition, it plays a role in early testicular development (PMID:31287541, PMID:31337883). It may also have an effect on brain development (PubMed:31256877). It is expressed in the fallopian tubes, ovaries, uterus, testes and brain (PMID: 31256877,PMID: 31337883).

Protocols

Product Specific Protocols
WB protocol for DHX37 antibody 29741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

DHX37 Is a Promising Prognostic Biomarker and a Therapeutic Target for Immunotherapy and Chemotherapy in HCC

Authors - Nanbin Liu
View Article