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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHX15 Recombinant monoclonal antibody (82137-1-RR)

    WB analysis using 82137-1-RR (same clone as 82137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82137-1-RR (DHX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHX15 Recombinant monoclonal antibody (82137-1-RR)

    WB analysis of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    WB result of DHX15 antibody (82137-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX15 transfected HeLa cells. This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using DHX15 Recombinant monoclonal antibody (82137-1-RR)

    IP experiment of NIH/3T3 using 82137-1-RR (same clone as 82137-1-PBS)

    IP result of anti-DHX15 (IP:82137-1-RR, 4ug; Detection:82137-1-RR 1:8000) with NIH/3T3 cells lysate 1320 ug. This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DHX15 Recombinant monoclonal antibody (82137-1-RR)

    IHC staining of mouse testis using 82137-1-RR (same clone as 82137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DHX15 Recombinant monoclonal antibody (82137-1-RR)

    IHC staining of mouse testis using 82137-1-RR (same clone as 82137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHX15 Recombinant monoclonal antibody (82137-1-RR)

    IF Staining of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHX15 Recombinant monoclonal antibody (82137-1-RR)

    IF Staining of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHX15 Recombinant monoclonal antibody (82137-1-RR)

    IF Staining of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DHX15 Recombinant monoclonal antibody (82137-1-RR)

    FC experiment of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DHX15 (82137-1-RR, Clone:1F13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82137-1-RR

    Affinity and Kinetic Characterization of 82137-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82137-1-RR against Human DHX15 were performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 82137-1-RR

    Affinity and Kinetic Characterization of 82137-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82137-1-RR against Human DHX15 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

DHX15 Recombinant monoclonal antibody, PBS Only

DHX15 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 82137-1-PBS
Clone No.1F13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

1F13, ATP-dependent RNA helicase #46, ATP-dependent RNA helicase DHX15, DBP1, DDX15

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82137-1-PBS targets DHX15 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2895

Product name: Recombinant human DHX15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 147-517 aa of BC035974

Sequence: TDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKL

相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 15
Calculated molecular weight 795 aa, 91 kDa
Observed molecular weight90-95 kDa
GenBank accession numberBC035974
Gene Symbol DHX15
Gene ID (NCBI) 1665
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43143
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kDa.

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