Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells, MCF-7 cells, HepG2 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86661-3-RR targets DHTKD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag26515 Product name: Recombinant human DHTKD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-197 aa of BC007955 Sequence: GYRPRKPESREPQGALERPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALLENVPEIQALVQTLQGPFHTAGLLNMGKEEASLEEVLVYLNQIYCGQISIETSQLQSQDEKDWFAKRFEELQKETFTTEERKHLSKLMLESQEFDHFLATKFSTVKRYGGEGAES 相同性解析による交差性が予測される生物種 |
| Full Name | dehydrogenase E1 and transketolase domain containing 1 |
| Calculated molecular weight | 103 kDa |
| Observed molecular weight | 103 kDa |
| GenBank accession number | BC007955 |
| Gene Symbol | DHTKD1 |
| Gene ID (NCBI) | 55526 |
| RRID | AB_3745037 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96HY7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DHTKD1 (Dehydrogenase E1 And Transketolase Domain Containing 1), also named as 2-aminoadipic 2-oxoadipic aciduria protein, OADC-E1, OADH-E1, is a lesser-studied E1 enzyme among the family of 2-oxoacid dehydrogenases (PMID: 32695416). DHTKD1 was expressed ubiquitously in various tissues and was proposed to localize to the mitochondria (PMID: 23141294). DHTKD1 plays a critical role in energy production in mitochondria, suppression of DHTKD1 leads to impaired mitochondrial biogenesis and increased cell apoptosis (PMID: 24076469).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DHTKD1 antibody 86661-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |