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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRSX Polyclonal antibody (17279-1-AP)

    WB analysis using 17279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17279-1-AP (DHRSX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DHRSX Polyclonal antibody (17279-1-AP)

    IP experiment of HeLa using 17279-1-AP

    IP result of anti-DHRSX (IP:17279-1-AP, 4ug; Detection:17279-1-AP 1:400) with HeLa cells lysate 1320 ug.

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DHRSX Polyclonal antibody

DHRSX Polyclonal Antibody for WB, IP, ELISA
Cat No. 17279-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

CXorf11, DHRS5X, DHRS5Y, DHRSX, DHRSXY, DHRSY, SDR46C1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17279-1-AP targets DHRSX in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRSX fusion protein Ag11046 相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) X-linked
Calculated molecular weight 330 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC032340
Gene Symbol DHRSX
Gene ID (NCBI) 207063
RRIDAB_3085520
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5I4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHRSX(Dehydrogenase/reductase SDR family member on chromosome X) is also named as CXorf11, DHRS5X and belongs to the short-chain dehydrogenases/reductases (SDR) family. It is involved in the metabolism of a large variety of compounds, including steroid hormones, prostaglandins, retinoids, lipids and xenobiotics.

Protocols

Product Specific Protocols
WB protocol for DHRSX antibody 17279-1-APDownload protocol
IP protocol for DHRSX antibody 17279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols