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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS9 Polyclonal antibody (14560-1-AP)

    WB analysis using 14560-1-AP (same clone as 14560-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHRS9 Polyclonal antibody (14560-1-AP)

    WB analysis of HeLa using 14560-1-AP (same clone as 14560-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse trachea tissue using DHRS9 Polyclonal antibody (14560-1-AP)

    WB analysis of mouse trachea using 14560-1-AP (same clone as 14560-1-PBS)

    mouse trachea tissue were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DHRS9 Polyclonal antibody (14560-1-AP)

    IHC staining of human lung cancer using 14560-1-AP (same clone as 14560-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DHRS9 Polyclonal antibody (14560-1-AP)

    IHC staining of human lung cancer using 14560-1-AP (same clone as 14560-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DHRS9 Polyclonal antibody (14560-1-AP)

    IF Staining of A549 using 14560-1-AP (same clone as 14560-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using DHRS9 antibody (14560-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DHRS9 Polyclonal antibody (14560-1-AP)

    IF Staining of A549 using 14560-1-AP (same clone as 14560-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 14560-1-AP (DHRS9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

DHRS9 Polyclonal antibody, PBS Only

DHRS9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14560-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14560-1-PBS targets DHRS9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6116

Product name: Recombinant human DHRS9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-319 aa of BC058883

Sequence: MLFWVLGLLILCGFLWTRKGKLKIEDITDKYIFITGCDSGFGNLAARTFDKKGFHVIAACLTESGSTALKAETSERLRTVLLDVTDPENVKRTAQWVKNQVGEKGLWGLINNAGVPGVLAPTDWLTLEDYREPIEVNLFGLISVTLNMLPLVKKAQGRVINVSSVGGRLAIVGGGYTPSKYAVEGFNDSLRRDMKAFGVHVSCIEPGLFKTNLADPVKVIEKKLAIWEQLSPDIKQQYGEGYIEKSLDKLKGNKSYVNMDLSPVVECMDHALTSLFPKTHYAAGKDAKIFWIPLSHMPAALQDFLLLKQKAELANPKAV

相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) member 9
Calculated molecular weight 35 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC058883
Gene Symbol DHRS9
Gene ID (NCBI) 10170
RRIDAB_2277277
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPW9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Dehydrogenase/reductase 9(DHRS9), is a member of the short-chain dehydrogenases/reductases (SDR) family. DHRS9 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. DHRS9 has 4 isoforms with molecular weight of 19, 30, 35, 42 kDa, and demonstrates oxidoreductase activity toward hydroxysteroids. DHRS9 is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus.

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