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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS7B Polyclonal antibody (15530-1-AP)

    WB analysis using 15530-1-AP (same clone as 15530-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15530-1-AP (DHRS7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15530-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DHRS7B Polyclonal antibody (15530-1-AP)

    IHC staining of mouse colon using 15530-1-AP (same clone as 15530-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15530-1-AP (DHRS7B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15530-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DHRS7B Polyclonal antibody (15530-1-AP)

    IHC staining of mouse colon using 15530-1-AP (same clone as 15530-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15530-1-AP (DHRS7B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15530-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DHRS7B Polyclonal antibody (15530-1-AP)

    IF Staining of U2OS using 15530-1-AP (same clone as 15530-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using DHRS7B antibody (15530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15530-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DHRS7B Polyclonal antibody (15530-1-AP)

    IF Staining of A431 using 15530-1-AP (same clone as 15530-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DHRS7B antibody (15530-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15530-1-PBS in a different storage buffer formulation.

DHRS7B Polyclonal antibody, PBS Only

DHRS7B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15530-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CGI 93, CGI-93, EC:1.1.-.-, Protein SDR32C1, SDR32C1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15530-1-PBS targets DHRS7B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7690

Product name: Recombinant human DHRS7B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 145-325 aa of BC004126

Sequence: ISYRGTIMDTTVDVDKRVMETNYFGPVALTKALLPSMIKRRQGHIVAISSIQGKMSIPFRSAYAASKHATQAFFDCLRAEMEQYEIEVTVISPGYIHTNLSVNAITADGSRYGVMDTTTAQGRSPVEVAQDVLAAVGKKKKDVILADLLPSLAVYLRTLAPGLFFSLMASRARKERKSKNS

相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) member 7B
Calculated molecular weight 35 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC004126
Gene Symbol DHRS7B
Gene ID (NCBI) 25979
RRIDAB_3741866
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6IAN0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DHRS7B (Dehydrogenase/Reductase 7B) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily, a group of enzymes involved in redox reactions, particularly the metabolism of steroids, lipids, and xenobiotics. DHRS7B is characterized by its NAD(P)H-dependent oxidoreductase activity and plays roles in cellular detoxification, lipid homeostasis, and possibly steroid hormone regulation.

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