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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS7 Polyclonal antibody (15061-1-AP)

    WB analysis using 15061-1-AP (same clone as 15061-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 15061-1-AP (DHRS7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using DHRS7 Polyclonal antibody (15061-1-AP)

    IHC staining of rat skeletal muscle using 15061-1-AP (same clone as 15061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15061-1-AP (DHRS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DHRS7 Polyclonal antibody (15061-1-AP)

    IHC staining of mouse skeletal muscle using 15061-1-AP (same clone as 15061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15061-1-AP (DHRS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15061-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DHRS7 Polyclonal antibody (15061-1-AP)

    IF Staining of HepG2 using 15061-1-AP (same clone as 15061-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DHRS7 antibody (15061-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 15061-1-PBS in a different storage buffer formulation.

DHRS7 Polyclonal antibody, PBS Only

DHRS7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15061-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CGI 86, CGI-86, DHRS7A, EC:1.1.1.-, Protein SDR34C1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15061-1-PBS targets DHRS7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7064

Product name: Recombinant human DHRS7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-183 aa of BC000637

Sequence: RADGDLTLLWAEWQGRRPEWELTDMVVWVTGASSGIGEELAYQLSKLGVSLVLSARRVHELERVKRRCLENGNLKEKDILVLPLDLTDTGSHEAATKAVLQEFGRIDILVNNGGMSQRSLCMDTSLDVYRKLIELNYLGTVSLTKCVLPHMIERKQG

相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) member 7
Calculated molecular weight 38 kDa
Observed molecular weight 32-38 kDa
GenBank accession numberBC000637
Gene Symbol DHRS7
Gene ID (NCBI) 51635
RRIDAB_3741858
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y394
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DHRS7 (Dehydrogenase/Reductase (SDR family) member 7), also known as SDR34C1, retSDR4, is a member of a large family of short-chain dehydrogenases/reductases (SDR). The DHRS7 gene codes for two subtypes and is found on chromosome 14. Isotype 1 (38 kDa) has 339 amino acids, while isotype 2 (32 kDa) contains 289 amino acids (PMID: 26466768). DHRS7 has been demonstrated to be an integral protein facing the lumen of the endoplasmic reticulum with lack of posttranscriptional glycosylation modification(PMID: 24246760).

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