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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis of rat liver using 15279-1-AP (same clone as 15279-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis using 15279-1-AP (same clone as 15279-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis of mouse liver using 15279-1-AP (same clone as 15279-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis of HeLa using 15279-1-AP (same clone as 15279-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DHRS4 Polyclonal antibody (15279-1-AP)

    WB analysis of HepG2 using 15279-1-AP (same clone as 15279-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IP experiment of mouse liver using 15279-1-AP (same clone as 15279-1-PBS)

    IP result of anti-DHRS4 (IP:15279-1-AP, 4ug; Detection:15279-1-AP 1:400) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IHC staining of human cervical cancer using 15279-1-AP (same clone as 15279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervix tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IHC staining of human cervix using 15279-1-AP (same clone as 15279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervix using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervix tissue using DHRS4 Polyclonal antibody (15279-1-AP)

    IHC staining of human cervix using 15279-1-AP (same clone as 15279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervix using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15279-1-PBS in a different storage buffer formulation.

DHRS4 Polyclonal antibody, PBS Only

DHRS4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15279-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15279-1-PBS targets DHRS4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7398

Product name: Recombinant human DHRS4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-278 aa of BC003019

Sequence: MHKAGLLGLCARAWNSVRMASSGMTRRDPLANKVALVTASTDGIGFAIARRLAQDGAHVVVSSRKQQNVDQAVATLQGEGLSVTGTVCHVGKAEDRERLVATAVKLHGGIDILVSNAAVNPFFGSIMDVTEEVWDKTLDINVKAPALMTKAVVPEMEKRGGGSVVIVSSIAAFSPSPGFSPYNVSKTALLGLTKTLAIELAPRNIRVNCLAPGLIKTSFSRMLWMDKEKEESMKETLRIRRLGEPEDCAGIVSFLCSEDASYITGETVVVGGGTPSRL

相同性解析による交差性が予測される生物種
Full Name dehydrogenase/reductase (SDR family) member 4
Calculated molecular weight 30 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC003019
Gene Symbol DHRS4
Gene ID (NCBI) 10901
RRIDAB_2292856
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTZ2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DHRS4(Dehydrogenase/reductase SDR family member 4) is also named as PHCR , NRDR ,PSCD , SCAD-SRL, and belongs to the short-chain dehydrogenases/reductases (SDR) family. It can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Human DHRS4 is a tetramer composed of 27 kDa subunits, which is inactivated at low temperature without dissociation into subunits(PMID:18571493).

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