Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, U2OS cells |
| Positive IHC detected in | Hepatocellular carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00088 targets DHRS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | dehydrogenase/reductase (SDR family) member 2 |
| Calculated molecular weight | 300 aa, 32 kDa |
| Observed molecular weight | 27-30 kDa |
| GenBank accession number | BC007339 |
| Gene Symbol | DHRS2 |
| Gene ID (NCBI) | 10202 |
| RRID | AB_3750137 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | Q13268 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DHRS2 antibody RMX00088 | Download protocol |
| IHC protocol for DHRS2 antibody RMX00088 | Download protocol |
| WB protocol for DHRS2 antibody RMX00088 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |