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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHPS Monoclonal antibody (67998-1-Ig)

    WB analysis using 67998-1-Ig (same clone as 67998-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67998-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using DHPS Monoclonal antibody (67998-1-Ig)

    WB analysis of LNCaP using 67998-1-Ig (same clone as 67998-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67998-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHPS Monoclonal antibody (67998-1-Ig)

    WB analysis of HeLa using 67998-1-Ig (same clone as 67998-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67998-1-PBS in a different storage buffer formulation.

DHPS Monoclonal antibody, PBS Only

DHPS Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67998-1-PBS
Clone No.1E4F5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

1E4F5, DHS, DS, EC:2.5.1.46, MIG13

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67998-1-PBS targets DHPS in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag31046

Product name: Recombinant human DHPS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-369 aa of BC014016

Sequence: MEGSLEREAPAGALAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEPLSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLVAETFAQKMDAFMHEKNED

相同性解析による交差性が予測される生物種
Full Name deoxyhypusine synthase
Calculated molecular weight 41 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014016
Gene Symbol DHPS
Gene ID (NCBI) 1725
RRIDAB_2918746
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP49366
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

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