Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, LNCaP cells |
| Positive IP detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
Product Information
11184-1-AP targets DHPS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1646 Product name: Recombinant human DHPS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-369 aa of BC014016 Sequence: MEGSLEREAPAGALAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEPLSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLVAETFAQKMDAFMHEKNED 相同性解析による交差性が予測される生物種 |
| Full Name | deoxyhypusine synthase |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC014016 |
| Gene Symbol | DHPS |
| Gene ID (NCBI) | 1725 |
| RRID | AB_2877751 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49366 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DHPS (Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. It functions as a homodimer and is essential for de novo folate synthesis in prokaryotes, lower eukaryotes, and plants, but is absent in mammals, which obtain folate from the diet. This absence makes DHPS an important target for selective antimicrobial agents, including sulfonamide drugs such as sulfamethoxazole and dapsone. Mutations in DHPS are associated with sulfonamide resistance in pathogens such as Pneumocystis jirovecii and Plasmodium falciparum. This protein has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DHPS antibody 11184-1-AP | Download protocol |
| IP protocol for DHPS antibody 11184-1-AP | Download protocol |
| WB protocol for DHPS antibody 11184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett Protective role of epithelial deoxyhypusine synthase in colorectal carcinogenesis caused by deletion of the adenomatous polyposis coli gene. | ||
BMC Med Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke. | ||
Amino Acids Epithelial hypusination regulates Helicobacter pylori-induced gastric inflammation. | ||
Res Sq Epithelial Hypusination Regulates Helicobacter pylori-induced Gastric Inflammation. | ||
Heliyon Analysis of the effect of hypusination in myeloid cells on colitis and colitis-associated cancer |