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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of LNCaP using 67977-1-Ig (same clone as 67977-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of HSC-T6 using 67977-1-Ig (same clone as 67977-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of NIH/3T3 using 67977-1-Ig (same clone as 67977-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of 4T1 using 67977-1-Ig (same clone as 67977-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of HeLa using 67977-1-Ig (same clone as 67977-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of HEK-293 using 67977-1-Ig (same clone as 67977-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of K-562 using 67977-1-Ig (same clone as 67977-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50416-1

    Cytometric bead array standard curve of MP50416-1

    Cytometric bead array standard curve of MP50416-1, DHODH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67977-2-PBS. Detection antibody: 67977-1-PBS. Standard:Ag6649. Range: 1.563-100 ng/mL.

DHODH Monoclonal antibody, PBS Only (Detector)

DHODH Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 67977-1-PBS
Clone No.2B2C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67977-1-PBS targets DHODH as part of a matched antibody pair:

MP50416-1: 67977-2-PBS capture and 67977-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6649

Product name: Recombinant human DHODH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-395 aa of BC065245

Sequence: MAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR

相同性解析による交差性が予測される生物種
Full Name dihydroorotate dehydrogenase
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC065245
Gene Symbol DHODH
Gene ID (NCBI) 1723
RRIDAB_2918726
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ02127
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

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