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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DHFRL1 Polyclonal antibody

DHFRL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27965-1-AP

Synonyms

DHFR2, DHFRL1, DHFRP4, Dihydrofolate reductase 2, mitochondrial, dihydrofolate reductase like 1, FLJ16119

Validation Data Gallery

Applications:
  • WB analysis using 27965-1-AP

    WB analysis using 27965-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27965-1-AP (DHFRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 27965-1-AP

    IHC staining of mouse heart using 27965-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 27965-1-AP

    IHC staining of mouse heart using 27965-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27965-1-AP targets DHFRL1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHFRL1 fusion protein Ag10537 相同性解析による交差性が予測される生物種
Full Name dihydrofolate reductase-like 1
Calculated molecular weight 187 aa, 22 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC063379
Gene symbol DHFRL1
Gene ID (NCBI) 200895
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHFRL1, also known as DHFR2, is a key enzyme in folate metabolism. DHFRL1 is a dihydrofolate reductase, that catalyzes the NADPH-dependent reduction of dihydrofolate to the biologically active form, tetrahydrofolate (PMID: 21876184).

Protocols

Product Specific Protocols
WB protocol for DHFRL1 antibody 27965-1-APDownload protocol
IHC protocol for DHFRL1 antibody 27965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols