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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHFR Monoclonal antibody (68274-1-Ig)

    WB analysis using 68274-1-Ig (same clone as 68274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using DHFR Monoclonal antibody (68274-1-Ig)

    WB analysis of NIH/3T3 using 68274-1-Ig (same clone as 68274-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using DHFR Monoclonal antibody (68274-1-Ig)

    FC experiment of MCF-7 using 68274-1-Ig (same clone as 68274-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug DHFR Monoclonal antibody (68274-1-Ig, Clone:4C11E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

DHFR Monoclonal antibody, PBS Only

DHFR Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68274-1-PBS
Clone No.4C11E9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

4C11E9, DHFRP1, EC:1.5.1.3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68274-1-PBS targets DHFR in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7318

Product name: Recombinant human DHFR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-187 aa of BC000192

Sequence: MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

相同性解析による交差性が予測される生物種
Full Name dihydrofolate reductase
Calculated molecular weight 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC000192
Gene Symbol DHFR
Gene ID (NCBI) 1719
RRIDAB_2935357
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP00374
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

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