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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DGKZ Recombinant antibody (84830-5-RR)

    WB analysis using 84830-5-RR (same clone as 84830-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84830-5-RR (DGKZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DGKZ Recombinant antibody (84830-5-RR)

    IHC staining of mouse brain using 84830-5-RR (same clone as 84830-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DGKZ Recombinant antibody (84830-5-RR)

    IHC staining of mouse brain using 84830-5-RR (same clone as 84830-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DGKZ Recombinant antibody (84830-5-RR)

    IHC staining of rat brain using 84830-5-RR (same clone as 84830-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using DGKZ Recombinant antibody (84830-5-RR)

    IHC staining of rat brain using 84830-5-RR (same clone as 84830-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84830-5-RR (DGKZ antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DGKZ Recombinant antibody (84830-5-RR)

    IF Staining of A431 using 84830-5-RR (same clone as 84830-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKZ antibody (84830-5-RR, Clone: 241444A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84830-5-PBS in a different storage buffer formulation.

DGKZ Recombinant monoclonal antibody, PBS Only

DGKZ Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84830-5-PBS
Clone No.241444A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

241444A2, DAG kinase zeta, DAGK5, DAGK6, DGK ZETA

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84830-5-PBS targets DGKZ in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag37336

Product name: Recombinant human DGKZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 750-982 aa of NM_001199267.1

Sequence: LDPELLGASARPDLPTPTSPLPTSPCSPTPRSLQGDAAPPQGEELIEAAKRNDFCKLQELHRAGGDLMHRDEQSRTLLHHAVSTGSKDVVRYLLDHAPPEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV

相同性解析による交差性が予測される生物種
Full Name diacylglycerol kinase, zeta 104kDa
Calculated molecular weight104kDa，928aa
Observed molecular weight 104-124 kDa
GenBank accession numberNM_001199267.1
Gene Symbol DGKZ
Gene ID (NCBI) 8525
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ13574
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Diacylglycerol kinase zeta (DGKZ), also known as DAGK5, DAGK6, DGK-ZETA, hDGKzeta, belongs to the diacylglycerol kinase (DGK) family which catalyzes diacylglycerol (DAG) into phosphatidic acid (PA). DGKZ is a novel promoter of metastasis and that it could be a potential prognostic indicator in patients with triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFbetaR2(PMID: 35115500). Downregulation of DGKZ suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest(PMID: 33926342).

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