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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using DGKQ Polyclonal antibody (17885-1-AP)

    WB analysis of mouse brain using 17885-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DGKQ Polyclonal antibody (17885-1-AP)

    WB analysis using 17885-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using DGKQ Polyclonal antibody (17885-1-AP)

    WB analysis of human placenta using 17885-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervix tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of human cervix using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 17885-1-AP (DGKQ antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of human cervix using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 17885-1-AP (DGKQ antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of human colon cancer using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of human colon cancer using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of mouse brain using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of mouse brain using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IF Staining of mouse small intestine using 17885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using DGKQ antibody (17885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IF Staining of mouse kidney using 17885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DGKQ antibody (17885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

DGKQ Polyclonal antibody

DGKQ Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 17885-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

DAGK, DAGK4, DAGK7, DGK theta, DGKtheta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, human placenta tissue, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human cervix tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17885-1-AP targets DGKQ in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11938

Product name: Recombinant human DGKQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 595-942 aa of BC063801

Sequence: PKSGGLKGRDLLCSFRKLLNPHQVFDLTNGGPLPGLHLFSQVPCFRVLVCGGDGTVGWVLGALEETRYRLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWTILLDAHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRLHNKGVYVRVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIFINIPSWGSGADLWGSDSDTRFEKPRMDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKVHMLRKAKQKPRRAGTTRDARADAAPAPESDPR

相同性解析による交差性が予測される生物種
Full Name diacylglycerol kinase, theta 110kDa
Calculated molecular weight 942 aa, 101 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC063801
Gene Symbol DGKQ
Gene ID (NCBI) 1609
RRIDAB_2091305
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP52824
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for DGKQ antibody 17885-1-APDownload protocol
IHC protocol for DGKQ antibody 17885-1-APDownload protocol
WB protocol for DGKQ antibody 17885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.

Authors - Jingjing Zhang
  • KO Validated
View Article
WB

J Sci Food Agric

Neonatal resveratrol administration promotes skeletal muscle growth and insulin sensitivity in intrauterine growth-retarded suckling piglets associated with activation of FGF21-AMPKα pathway

Authors - Guangyi Bai
View Article
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