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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DFFA,DFF45 Recombinant antibody (85220-5-RR)

    WB analysis using 85220-5-RR (same clone as 85220-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85220-5-RR (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85220-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DFFA,DFF45 Recombinant antibody (85220-5-RR)

    IF Staining of HeLa using 85220-5-RR (same clone as 85220-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DFFA,DFF45 antibody (85220-5-RR, Clone: 242915D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85220-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85220-5-RR

    Affinity and Kinetic Characterization of 85220-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85220-5-RR against Human DFFA,DFF45 were performed. The affinity constant is 1.47 nM.

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DFFA,DFF45 Recombinant antibody, PBS Only

DFFA,DFF45 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85220-5-PBS
Clone No.242915D6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DFF45, DFFA, DFF45/DFFA, DFF-45, DFF1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85220-5-PBS targets DFFA,DFF45 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DFFA,DFF45 fusion protein Ag0244 相同性解析による交差性が予測される生物種
Full Name DNA fragmentation factor, 45kDa, alpha polypeptide
Calculated molecular weight 37 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC000037
Gene Symbol DFFA
Gene ID (NCBI) 1676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00273
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.