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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DEPDC6 Polyclonal antibody (21312-1-AP)

    WB analysis of HeLa using 21312-1-AP (same clone as 21312-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using DEPDC6 Polyclonal antibody (21312-1-AP)

    WB analysis of COLO 320 using 21312-1-AP (same clone as 21312-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using DEPDC6 Polyclonal antibody (21312-1-AP)

    WB analysis of HT-1080 using 21312-1-AP (same clone as 21312-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DEPDC6 Polyclonal antibody (21312-1-AP)

    IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using DEPDC6 Polyclonal antibody (21312-1-AP)

    IHC staining of human thyroid cancer using 21312-1-AP (same clone as 21312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21312-1-PBS in a different storage buffer formulation.

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DEPDC6 Polyclonal antibody, PBS Only

DEPDC6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21312-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21312-1-PBS targets DEPDC6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEPDC6 fusion protein Ag15959 相同性解析による交差性が予測される生物種
Full Name DEP domain containing 6
Calculated molecular weight 409 aa, 46 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC024746
Gene Symbol DEPDC6
Gene ID (NCBI) 64798
RRIDAB_2878837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TB45
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.