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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using DENND1A Polyclonal antibody (25658-1-AP)

    WB analysis of SKOV-3 using 25658-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DENND1A Polyclonal antibody (25658-1-AP)

    WB analysis using 25658-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DENND1A Polyclonal antibody (25658-1-AP)

    WB analysis of K-562 using 25658-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DENND1A Polyclonal antibody (25658-1-AP)

    WB analysis of K-562 using 25658-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using DENND1A Polyclonal antibody (25658-1-AP)

    IP experiment of K-562 using 25658-1-AP

    IP result of anti-DENND1A (IP:25658-1-AP, 4ug; Detection:25658-1-AP 1:600) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using DENND1A Polyclonal antibody (25658-1-AP)

    IHC staining of human endometrial cancer using 25658-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using DENND1A Polyclonal antibody (25658-1-AP)

    IHC staining of human endometrial cancer using 25658-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using DENND1A Polyclonal antibody (25658-1-AP)

    IHC staining of human liver using 25658-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25658-1-AP (DENND1A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using DENND1A Polyclonal antibody (25658-1-AP)

    IHC staining of human liver using 25658-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25658-1-AP (DENND1A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using DENND1A Polyclonal antibody (25658-1-AP)

    IF Staining of SKOV-3 using 25658-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using DENND1A antibody (25658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DENND1A Polyclonal antibody

DENND1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25658-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Connecdenn, DENN/MADD domain containing 1A, DENND1A, FAM31A, KIAA1608, Protein FAM31A, RP11 230L22.3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSKOV-3 cells, A2780 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman endometrial cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25658-1-AP targets DENND1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22547

Product name: Recombinant human DENND1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 451-559 aa of BC039703

Sequence: QKDIAENGCAPTPEEQLPKTAPSPLVEAKDPKLREDRRPITVHFGQVRPPRPHVVKRPKSNIAVEGRRTSVPSPEQNTIATPATLHILQKSITHFAAKFPTRGWTSSSH

相同性解析による交差性が予測される生物種
Full Name DENN/MADD domain containing 1A
Calculated molecular weight 1009 aa, 111 kDa
Observed molecular weight 111 kDa, 64 kDa
GenBank accession numberBC039703
Gene Symbol DENND1A
Gene ID (NCBI) 57706
RRIDAB_2880180
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEH3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DENND1A (DENN/MADD domain containing 1A), also known as Connecdenn,FAM31A or KIAA1608, is a 1,009 amino acid peripheral membrane protein. Recent studies has found DENND1A is strongly associated with PCOS (Polycystic ovary syndrome). DENND1A has several isoforms.This antibody(25658-1-AP) can recognize all the isoforms at 112 kDa, 52-64 kDa.

Protocols

Product Specific Protocols
WB protocol for DENND1A antibody 25658-1-APDownload protocol
IHC protocol for DENND1A antibody 25658-1-APDownload protocol
IF protocol for DENND1A antibody 25658-1-APDownload protocol
IP protocol for DENND1A antibody 25658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols