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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DEM1/EXO5 Polyclonal antibody

DEM1/EXO5 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25991-1-AP

Synonyms

C1orf176, DEM1, EXO5, Exonuclease V

Validation Data Gallery

Applications:
  • WB analysis using 25991-1-AP

    WB analysis using 25991-1-AP

    NIH/3T3 cells lysates were subjected to SDS PAGE followed by western blot with 25991-1-AP (DEM1/EXO5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 25991-1-AP

    IHC staining of human tonsillitis using 25991-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25991-1-AP (DEM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 25991-1-AP

    IHC staining of human tonsillitis using 25991-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25991-1-AP (DEM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 25991-1-AP

    IF Staining of A431 using 25991-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DEM1/EXO5 antibody (25991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

25991-1-AP targets DEM1/EXO5 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEM1/EXO5 fusion protein Ag22666 相同性解析による交差性が予測される生物種
Full Name defects in morphology 1 homolog (S. cerevisiae)
Observed molecular weight42 kDa
GenBank accession numberBC021969
Gene symbol DEM1
Gene ID (NCBI) 64789
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
IHC protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
IF protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols