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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DEM1/EXO5 Polyclonal antibody (25991-1-AP)

    WB analysis using 25991-1-AP

    NIH/3T3 cells lysates were subjected to SDS PAGE followed by western blot with 25991-1-AP (DEM1/EXO5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DEM1/EXO5 Polyclonal antibody (25991-1-AP)

    IHC staining of mouse heart using 25991-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25991-1-AP (DEM1/EXO5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DEM1/EXO5 Polyclonal antibody (25991-1-AP)

    IHC staining of mouse heart using 25991-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25991-1-AP (DEM1/EXO5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DEM1/EXO5 Polyclonal antibody (25991-1-AP)

    IHC staining of human tonsillitis using 25991-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25991-1-AP (DEM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DEM1/EXO5 Polyclonal antibody (25991-1-AP)

    IHC staining of human tonsillitis using 25991-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25991-1-AP (DEM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DEM1/EXO5 Polyclonal antibody (25991-1-AP)

    IF Staining of A431 using 25991-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DEM1/EXO5 antibody (25991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DEM1/EXO5 Polyclonal antibody

DEM1/EXO5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25991-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DEM1, EXO5, C1orf176, EC:3.1.-.-, Exo V

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25991-1-AP targets DEM1/EXO5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22666

Product name: Recombinant human DEM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-373 aa of BC021969

Sequence: MAETREEETVSAEASGFSDLSDSEFLEFLDLEDAQESKALVNMPGPSSESLGKDDKPISLQNWKRGLDILSPMERFHLKYLYVTDLATQNWCELQTAYGKELPGFLAPEKAAVLDTGASIHLARELELHDLVTVPVTTKEDAWAIKFLNILLLIPTLQSEGHIREFPVFGEVEGVLLVGVIDELHYTAKGELELAELKTRRRPMLPLEAQKKKDCFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLEKPLGPSVLRHAQQGGFSVKSLGDLMELVFLSLTLSDLPVIDILKIEYIHQETATVLGTEIVAFKEKEVRAKVQHYMAYWMGHREPQGVDVEEAWKCRTCTYADICEWRKGSGVLSSTLAPQVKKAK

相同性解析による交差性が予測される生物種
Full Name defects in morphology 1 homolog (S. cerevisiae)
Observed molecular weight42 kDa
GenBank accession numberBC021969
Gene Symbol DEM1
Gene ID (NCBI) 64789
RRIDAB_3085832
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
IHC protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
WB protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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