Validation Data Gallery
Tested Applications
| Positive IP detected in | LNCaP cells |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33219-1-AP targets DEFB132 in IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37893 Product name: Recombinant human DEFB132 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-95 aa of BC137293 Sequence: GGSKCVSNTPGYCRTCCHWGETALFMCNASRKCCISYSFLPKPDLPQLIGNHWQSRRRNTQRKDKKQQTTVTS 相同性解析による交差性が予測される生物種 |
| Full Name | defensin, beta 132 |
| Observed molecular weight | 11 kDa |
| GenBank accession number | BC137293 |
| Gene Symbol | DEFB132 |
| Gene ID (NCBI) | 400830 |
| RRID | AB_3742901 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q7Z7B7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human DEFB132 (Defensin Beta 132) is a member of the beta-defensin family and functions as a secreted antimicrobial peptide. This protein is highly expressed in epididymal epithelial cells and sperm, regulated by the androgen receptor signaling pathway, and serves as a key effector molecule of innate immunity in the male reproductive tract. DEFB132 exhibits broad-spectrum antimicrobial activity, inhibiting both Gram-negative bacteria such as *Escherichia coli* and Gram-positive bacteria such as *Staphylococcus aureus*, exerting its bactericidal function by disrupting the cell membranes of pathogens. As an inducible immune effector molecule, it plays multiple biological roles in host defense, reproductive health, and metabolic homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DEFB132 antibody 33219-1-AP | Download protocol |
| IP protocol for DEFB132 antibody 33219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |