• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DEFA5 Polyclonal antibody

DEFA5 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18268-1-AP

Synonyms

DEF5, DEFA5, Defensin 5, Defensin, alpha 5, defensin, alpha 5, Paneth cell, HD 5

Validation Data Gallery

Applications:
  • IHC staining of human small intestine using 18268-1-AP

    IHC staining of human small intestine using 18268-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18268-1-AP (DEFA5 antibody) at dilution of 1:3200 (under 10x lens)..

  • IHC staining of human small intestine using 18268-1-AP

    IHC staining of human small intestine using 18268-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18268-1-AP (DEFA5 antibody) at dilution of 1:3200 (under 40x lens)..

Tested Applications

Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18268-1-AP is DEFA5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEFA5 fusion protein Ag12793 相同性解析による交差性が予測される生物種
Full Name defensin, alpha 5, Paneth cell-specific
Calculated molecular weight 94 aa, 10 kDa
GenBank accession numberBC069690
Gene symbol DEFA5
Gene ID (NCBI) 1670
RRIDAB_2878526
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DEFA5, also named as DEF5, belongs to the alpha-defensin family. It has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Mature DEFA5 is about 5kd. It has a homodimer form.

Protocols

Product Specific Protocols
IHC protocol for DEFA5 antibody 18268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols