Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of human peripheral blood leukocyte using 67156-1-Ig (same clone as 67156-1-PBS)
Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 67156-1-Ig (DEFA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.
IHC staining of human small intestine using 67156-1-Ig (same clone as 67156-1-PBS)
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.
IHC staining of human small intestine using 67156-1-Ig (same clone as 67156-1-PBS)
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.
IF Staining of mouse spleen using 67156-1-Ig (same clone as 67156-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using DEFA1 antibody (67156-1-Ig, Clone: 1A2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using DEFA1 antibody (67156-1-Ig, Clone: 1A2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67156-1-PBS in a different storage buffer formulation.

DEFA1 Monoclonal antibody, PBS Only

DEFA1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67156-1-PBS

Clone No.1A2B3

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

1A2B3, DEFA 1, DEFA1B, DEFA2, defensin, alpha 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

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MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67156-1-PBS targets DEFA1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag12592 Product name: Recombinant human DEFA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-94 aa of BC093791 Sequence: MRTLAILAAILLVALQAQAEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMACYCRIPACIAGERRYGTCIYQGRLWAFCC 相同性解析による交差性が予測される生物種
Full Name	defensin, alpha 1
Calculated molecular weight	94 aa, 10 kDa
Observed molecular weight	6-10 kDa
GenBank accession number	BC093791
Gene Symbol	DEFA1
Gene ID (NCBI)	1667
RRID	AB_2882453
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P59665
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

製品

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サポート

カスタム対応

プロモーション

企業情報

抗体

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研究分野

Target

Host

Species Reactivity