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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

DEF6 Polyclonal antibody

DEF6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11369-1-AP

Synonyms

DEF 6, DEF6, IBP, IRF4 binding protein, SLAT

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 11369-1-AP

    WB analysis of Jurkat using 11369-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11369-1-AP (DEF6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 11369-1-AP

    WB analysis of Jurkat using 11369-1-AP

    WB result of DEF6 antibody (11369-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEF6 transfected Jurkat cells.

  • IP experiment of Jurkat using 11369-1-AP

    IP experiment of Jurkat using 11369-1-AP

    IP result of anti-DEF6 (IP:11369-1-AP, 4ug; Detection:11369-1-AP 1:600) with Jurkat cells lysate 2400ug.

  • IHC staining of mouse spleen using 11369-1-AP

    IHC staining of mouse spleen using 11369-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11369-1-AP (DEF6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEF6 antibody included
  • IHC staining of mouse spleen using 11369-1-AP

    IHC staining of mouse spleen using 11369-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11369-1-AP (DEF6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEF6 antibody included
  • IHC staining of human tonsillitis using 11369-1-AP

    IHC staining of human tonsillitis using 11369-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11369-1-AP (DEF6 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with DEF6 antibody included
  • IHC staining of human tonsillitis using 11369-1-AP

    IHC staining of human tonsillitis using 11369-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11369-1-AP (DEF6 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with DEF6 antibody included

Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DEF6 Ready-To-Use IHC Kit. DEF6 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11369-1-AP targets DEF6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEF6 fusion protein Ag1925 相同性解析による交差性が予測される生物種
Full Name differentially expressed in FDCP 6 homolog (mouse)
Calculated molecular weight 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC017504
Gene symbol DEF6
Gene ID (NCBI) 50619
RRIDAB_2230504
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DEF6 antibody 11369-1-APDownload protocol
IHC protocol for DEF6 antibody 11369-1-APDownload protocol
IP protocol for DEF6 antibody 11369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols