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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX60 Recombinant antibody

DDX60 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

230293C10

Cat no : 82969-1-RR

Synonyms

DDX60, DEAD box protein 60, FLJ10787, FLJ20035

Validation Data Gallery

Applications:
  • WB analysis using 82969-1-RR

    WB analysis using 82969-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82969-1-RR (DDX60 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 82969-1-RR

    IF Staining of A431 using 82969-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DDX60 antibody (82969-1-RR, Clone: 230293C10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A431 using 82969-1-RR

    IF Staining of A431 using 82969-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DDX60 antibody (82969-1-RR, Clone: 230293C10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, U-87 MG cells, U-251 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:125-1:500
Sample-dependent, check data in validation data gallery

Product Information

82969-1-RR targets DDX60 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX60 fusion protein Ag34406 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Calculated molecular weight 1712 aa, 198 kDa
Observed molecular weight170-200 kDa
GenBank accession numberBC038115
Gene symbol DDX60
Gene ID (NCBI) 55601
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

Protocols

Product Specific Protocols
WB protocol for DDX60 antibody 82969-1-RRDownload protocol
IF protocol for DDX60 antibody 82969-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols