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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX54 Monoclonal antibody (66664-1-Ig)

    WB analysis using 66664-1-Ig (same clone as 66664-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66664-1-Ig (DDX54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DDX54 Monoclonal antibody (66664-1-Ig)

    WB analysis using 66664-1-Ig (same clone as 66664-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66664-1-Ig (DDX54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DDX54 Monoclonal antibody (66664-1-Ig)

    IF Staining of PC-3 using 66664-1-Ig (same clone as 66664-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (66664-1-Ig, Clone: 1H9F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DDX54 Monoclonal antibody (66664-1-Ig)

    IF Staining of PC-3 using 66664-1-Ig (same clone as 66664-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (66664-1-Ig, Clone: 1H9F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using DDX54 Monoclonal antibody (66664-1-Ig)

    FC experiment of PC-3 using 66664-1-Ig (same clone as 66664-1-PBS)

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human DDX54 (66664-1-Ig, Clone:1H9F4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

DDX54 Monoclonal antibody, PBS Only

DDX54 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66664-1-PBS
Clone No.1H9F4

Host / Isotype

Mouse / IgG2a

Reactivity

mouse, human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EC:3.6.4.13, DP97, DDX 54, ATP-dependent RNA helicase DP97, ATP-dependent RNA helicase DDX54

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66664-1-PBS targets DDX54 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25289

Product name: Recombinant human DDX54 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 778-881 aa of BC156669

Sequence: DDRDSDEEGASDRRGPERRGGKRDRGQGASRPHAPGTPAGRVRPELKTKQQILKQRRRAQKLHFLQRGGLKQLSARNRRRVQELQQGAFGRGARSKKGKMRKRM

相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Calculated molecular weight 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC156669
Gene Symbol DDX54
Gene ID (NCBI) 79039
RRIDAB_2882019
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8TDD1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DDX54, also named as ATP-dependent RNA helicase DDX54, is a 881 amino acid protein, which contains 1 helicase ATP-binding domain and belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. DDX54 localizes in nucleus and Interacts in a hormone-dependent manner with nuclear receptors. DDX54 has RNA-dependent ATPase activity and represses the transcriptional activity of nuclear receptors.

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