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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DDX49 Polyclonal antibody (20523-1-AP)

    WB analysis of HeLa using 20523-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20523-1-AP (DDX49 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using DDX49 Polyclonal antibody (20523-1-AP)

    WB analysis of Y79 using 20523-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 20523-1-AP (DDX49 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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DDX49 Polyclonal antibody

DDX49 Polyclonal Antibody for WB, ELISA
Cat No. 20523-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DDX49, DEAD box protein 49, R27090_2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20523-1-AP targets DDX49 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX49 fusion protein Ag14373 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 49
Calculated molecular weight 483 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC002674
Gene Symbol DDX49
Gene ID (NCBI) 54555
RRIDAB_2878698
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6V7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DDX49 antibody 20523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols