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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX47 Recombinant antibody (83287-3-RR)

    WB analysis using 83287-3-RR (same clone as 83287-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83287-3-RR (DDX47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DDX47 Recombinant antibody (83287-3-RR)

    IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DDX47 Recombinant antibody (83287-3-RR)

    IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using DDX47 Recombinant antibody (83287-3-RR)

    FC experiment of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX47 Recombinant antibody (83287-3-RR, Clone:240193C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

DDX47 Recombinant monoclonal antibody, PBS Only

DDX47 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83287-3-PBS
Clone No.240193C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

MSTP162, HQ0256, EC:3.6.4.13, E4 DBP, DEAD box protein 47

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83287-3-PBS targets DDX47 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11953

Product name: Recombinant human DDX47 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 202-455 aa of BC068009

Sequence: FLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR

相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
Calculated molecular weight 455 aa, 51 kDa
GenBank accession numberBC068009
Gene Symbol DDX47
Gene ID (NCBI) 51202
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9H0S4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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