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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX47 Polyclonal antibody

DDX47 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17795-1-AP

Synonyms

DDX47, DEAD box protein 47, E4 DBP, HQ0256, MSTP162

Validation Data Gallery

Applications:
  • WB analysis using 17795-1-AP

    WB analysis using 17795-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17795-1-AP (DDX47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 17795-1-AP

    IF Staining of U2OS using 17795-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (17795-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, SH-SY5Y cells
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

17795-1-AP targets DDX47 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX47 fusion protein Ag11953 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
Calculated molecular weight 455 aa, 51 kDa
Observed molecular weight48 kDa
GenBank accession numberBC068009
Gene symbol DDX47
Gene ID (NCBI) 51202
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).

Protocols

Product Specific Protocols
WB protocol for DDX47 antibody 17795-1-APDownload protocol
IF protocol for DDX47 antibody 17795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols