Validation Data Gallery
Tested Applications
| Positive WB detected in | Calu-3 cells, LNCaP cells, HeLa cells, U2OS cells, DU 145 cells, NCCIT cells, rabbit testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68454-1-Ig targets DDX43 in WB, ELISA applications and shows reactivity with Human, Rat, Rabbit samples.
| Tested Reactivity | Human, Rat, Rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11484 Product name: Recombinant human DDX43 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 264-648 aa of BC066938 Sequence: KPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCYLMPGFIHLVLQPSLKGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRSVCVYGGGNRDEQIEELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEPQIMKILLDVRPDRQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVTTEEEKWSHMQTFLQSMSSTDKVIVFVSRKAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRILIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRTGVSITTLTRNDWRVASELINILERANQSIPEELVSMAERFKAHQQKREMERKMERPQGRPKKFH 相同性解析による交差性が予測される生物種 |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 |
| Calculated molecular weight | 648 aa, 73 kDa |
| Observed molecular weight | 68-73 kDa |
| GenBank accession number | BC066938 |
| Gene Symbol | DDX43 |
| Gene ID (NCBI) | 55510 |
| RRID | AB_3085166 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NXZ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. DDX43 is highly expressed in many tumors and is, therefore, considered a potential target for immunotherapy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX43 antibody 68454-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |