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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX39A Recombinant antibody

DDX39A Recombinant Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230375F9

Cat no : 83083-5-RR

Synonyms

BAT1, BAT1L, DDX39, DDXL, DEAD box protein 39, Nuclear RNA helicase URH49, URH49

Validation Data Gallery

Applications:
  • WB analysis using 83083-5-RR

    WB analysis using 83083-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83083-5-RR

    WB analysis using 83083-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83083-5-RR

    IF Staining of HepG2 using 83083-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX39 antibody (83083-5-RR, Clone: 230375F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of Jurkat using 83083-5-RR

    FC experiment of Jurkat using 83083-5-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human DDX39A (83083-5-RR, Clone:230375F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inJurkat cells, mouse kidney tissue, HeLa cells, HepG2 cells, Raji cells, HEK-293 cells, A431 cells
Positive IF detected inHepG2 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

83083-5-RR targets DDX39A in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX39A fusion protein Ag2311 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
Calculated molecular weight 427 aa, 49 kDa
Observed molecular weight50 kDa
GenBank accession numberBC032128
Gene symbol DDX39
Gene ID (NCBI) 10212
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA-binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs. Some of the motifs were known before the definition of the family and are responsible for binding to mRNA or ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes.

Protocols

Product Specific Protocols
WB protocol for DDX39A antibody 83083-5-RRDownload protocol
IF protocol for DDX39A antibody 83083-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols