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Target

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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

DDX3 Polyclonal antibody

DDX3 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, RIP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11115-1-AP

Synonyms

DBX, DDX14, DDX3, DDX3X, DEAD box, X isoform, Helicase like protein 2, HLP2

Validation Data Gallery

Applications:
  • WB analysis using 11115-1-AP

    WB analysis using 11115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11115-1-AP

    WB analysis using 11115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 11115-1-AP

    WB analysis of HepG2 using 11115-1-AP

    WB result of DDX3 antibody (11115-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells.

  • WB analysis of HeLa using 11115-1-AP

    WB analysis of HeLa using 11115-1-AP

    WB result of DDX3 antibody (11115-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HeLa cells.

  • WB analysis using 11115-1-AP

    WB analysis using 11115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 11115-1-AP

    WB analysis of NIH/3T3 using 11115-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HT-1080 using 11115-1-AP

    WB analysis of HT-1080 using 11115-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 11115-1-AP

    WB analysis of mouse liver using 11115-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11115-1-AP (DDX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 11115-1-AP

    IP experiment of HeLa using 11115-1-AP

    IP result of anti-DDX3 (IP:11115-1-AP, 4ug; Detection:11115-1-AP 1:300) with HeLa cells lysate 2480ug.

  • IHC staining of human malignant melanoma using 11115-1-AP

    IHC staining of human malignant melanoma using 11115-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 11115-1-AP (DDX3 antibody) at dilution of 1:200 (under 40x lens)..

    Ready-to-use IHC kits available with DDX3 antibody included
  • IHC staining of human malignant melanoma using 11115-1-AP

    IHC staining of human malignant melanoma using 11115-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 11115-1-AP (DDX3 antibody) at dilution of 1:200 (under 10x lens)..

    Ready-to-use IHC kits available with DDX3 antibody included
  • IHC staining of human cervical cancer using 11115-1-AP

    IHC staining of human cervical cancer using 11115-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11115-1-AP (DDX3 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with DDX3 antibody included
  • IF Staining of HepG2 using 11115-1-AP

    IF Staining of HepG2 using 11115-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (11115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 11115-1-AP

    IF Staining of HepG2 using 11115-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX3 antibody (11115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HepG2 using 11115-1-AP

    IF Staining of HepG2 using 11115-1-AP

    Immunofluorescent analysis of HepG2 cells using 11115-1-AP (DDX3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • FC experiment of HeLa using 11115-1-AP

    FC experiment of HeLa using 11115-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DDX3 (11115-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, mouse liver tissue, HT-1080 cells, mouse brain tissue, PC-12 cells, K-562 cells, C6 cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman malignant melanoma tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DDX3 Ready-To-Use IHC Kit. DDX3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

11115-1-AP targets DDX3 in WB, IP, IF, FC, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX3 fusion protein Ag1614 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Calculated molecular weight 73 kDa
Observed molecular weight73 kDa
GenBank accession numberBC011819
Gene symbol DDX3X
Gene ID (NCBI) 1654
RRIDAB_10896499
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

Protocols

Product Specific Protocols
WB protocol for DDX3 antibody 11115-1-APDownload protocol
IHC protocol for DDX3 antibody 11115-1-APDownload protocol
IF protocol for DDX3 antibody 11115-1-APDownload protocol
IP protocol for DDX3 antibody 11115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Neuron

Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development.

Authors - Ashley L Lennox
View Article
humanWB

Neuron

CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG Translation.

Authors - Weiwei Cheng
  • KD Validated
View Article
humanWB

Pharmacol Res

HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5

Authors - Fu-Chun Huo
  • KD Validated
View Article
mouseWB,IF,CoIP

Ecotoxicol Environ Saf

Aluminum exposure induces central nervous system impairment via activating NLRP3-medicated pyroptosis pathway

Authors - Wudi Hao
View Article
WB,IF

J Virol

DDX3X Is Hijacked by Snakehead Vesiculovirus Phosphoprotein To Facilitate Virus Replication via Stabilization of the Phosphoprotein

Authors - Congran Bei
View Article
humanWB,IF

Oncotarget

(DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit.

Authors - Xin Wang
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Shannon (Verified Customer) (07-19-2022)

Worked well, also binds to different isoforms/protein dimers

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Jurkat T-Cells
DDX3 Antibody Western Blot,Immunoprecipitation validation (1:2000 dilution) in Jurkat T-Cells (Cat no:11115-1-AP)