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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX24 Recombinant antibody (84103-6-RR)

    WB analysis using 84103-6-RR (same clone as 84103-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84103-6-RR (DDX24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84103-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX24 Recombinant antibody (84103-6-RR)

    IF Staining of HeLa using 84103-6-RR (same clone as 84103-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX24 antibody (84103-6-RR, Clone: 241145G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84103-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using DDX24 Recombinant antibody (84103-6-RR)

    FC experiment of U-2 OS using 84103-6-RR (same clone as 84103-6-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug DDX24 Recombinant antibody (84103-6-RR, Clone:241145G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84103-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84103-6-RR

    Affinity and Kinetic Characterization of 84103-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84103-6-RR against Human DDX24 were performed. The affinity constant is below 1 pM.

DDX24 Recombinant monoclonal antibody, PBS Only

DDX24 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84103-6-PBS
Clone No.241145G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24, 241145G10

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84103-6-PBS targets DDX24 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8407

Product name: Recombinant human DDX24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 509-859 aa of BC008847

Sequence: TLTLVHQAPARILHKKHTKKMDKTAKLDLLMQKIGMRGKPKVIDLTRNEATVETLTETKIHCETDEKDFYLYYFLMQYPGRSLVFANSISCIKRLSGLLKVLDIMPLTLHACMHQKQRLRNLEQFARLEDCVLLATDVAARGLDIPKVQHVIHYQVPRTSEIYVHRSGRTARATNEGLSLMLIGPEDVINFKKIYKTLKKDEDIPLFPVQTKYMDVVKERIRLARQIEKSEYRNFQACLHNSWIEQAAAALEIELEEDMYKGGKADQQEERRRQKQMKVLKKELRHLLSQPLFTESQKTKYPTQSGKPPLLVSAPSKSESALSCLSKQKKKKTKKPKEPQPEQPQPSTSAN

相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
Calculated molecular weight 96 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC008847
Gene Symbol DDX24
Gene ID (NCBI) 57062
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9GZR7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.

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