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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using DDX23 Polyclonal antibody (10199-2-AP)

    WB analysis of K-562 using 10199-2-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10199-2-AP (DDX23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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DDX23 Polyclonal antibody

DDX23 Polyclonal Antibody for WB, ELISA
Cat No. 10199-2-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DDX23, DEAD box protein 23, prp28, PRP28 homolog, PRPF28, U5 100K, U5 100KD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10199-2-AP targets DDX23 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX23 fusion protein Ag0258 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
Calculated molecular weight 96 kDa
Observed molecular weight 96-100 kDa
GenBank accession numberBC002366
Gene Symbol DDX23
Gene ID (NCBI) 9416
RRIDAB_2261707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 (DDX23, synonyms: prp28, MGC8416, U5-100K) is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX23 is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.

Protocols

Product Specific Protocols
WB protocol for DDX23 antibody 10199-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

N 2-Alkyl-dG lesions elicit R-loop accumulation in the genome

Authors - Yinan Wang
View Article