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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX21 Recombinant antibody (80932-1-RR)

    WB analysis using 80932-1-RR (same clone as 80932-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80932-1-RR (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DDX21 Recombinant antibody (80932-1-RR)

    WB analysis of HeLa using 80932-1-RR (same clone as 80932-1-PBS)

    WB result of DDX21 antibody (80932-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX21 transfected HeLa cells. This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX21 Recombinant antibody (80932-1-RR)

    IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDX21 Recombinant antibody (80932-1-RR)

    IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80932-1-RR

    Affinity and Kinetic Characterization of 80932-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80932-1-RR against Human DDX21 were performed. The affinity constant is 77.7 nM.

  • Featured Product
  • KD/KO Validated

DDX21 Recombinant monoclonal antibody, PBS Only

DDX21 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 80932-1-PBS
Clone No.5E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

5E7, DEAD box protein 21, DExD-box helicase 21, EC:3.6.4.13, Gu alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80932-1-PBS targets DDX21 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0804

Product name: Recombinant human DDX21 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-180 aa of BC008071

Sequence: MPGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIP

相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Calculated molecular weight 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC008071
Gene Symbol DDX21
Gene ID (NCBI) 9188
RRIDAB_2918920
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NR30
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa.