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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX11 Polyclonal antibody (13047-1-AP)

    WB analysis using 13047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13047-1-AP (DDX11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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DDX11 Polyclonal antibody

DDX11 Polyclonal Antibody for WB, ELISA
Cat No. 13047-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ATP-dependent DNA helicase DDX11, CHL1-related protein 1, DDX 11, DEAD/H-box protein 11, EC:3.6.4.12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13047-1-AP targets DDX11 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX11 fusion protein Ag3666 相同性解析による交差性が予測される生物種
Full Name DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae)
Calculated molecular weight 970 aa, 108 kDa
Observed molecular weight102-108 kDa
GenBank accession numberBC011264
Gene Symbol DDX11
Gene ID (NCBI) 1663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FC9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX11 (DEAD/H-box helicase 11), also known as hCHLR1, KRG-2, is a member of the DExD/H (Asp-Glu-x-Asp/His)-box helicase family containing DNA helicase with roles in DNA replication and sister chromatid cohesion establishment, and general chromosome architecture (PMID: 33802088). DDX11 encodes an iron-sulfur cluster DNA helicase required for development, mutated, and overexpressed in cancers (PMID: 33879618). DDX11 is also a positive regulator of the RIG-I-mitochondrial antiviral signaling protein (MAVS)-mediated signaling pathways (PMID: 39470258).

Protocols

Product Specific Protocols
WB protocol for DDX11 antibody 13047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols