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Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using DDX10 Polyclonal antibody (17857-1-AP)

    WB analysis of SMMC-7721 using 17857-1-AP (same clone as 17857-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DDX10 Polyclonal antibody (17857-1-AP)

    WB analysis of HeLa using 17857-1-AP (same clone as 17857-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DDX10 Polyclonal antibody (17857-1-AP)

    IHC staining of human kidney using 17857-1-AP (same clone as 17857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX10 Polyclonal antibody (17857-1-AP)

    IHC staining of mouse testis using 17857-1-AP (same clone as 17857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDX10 Polyclonal antibody (17857-1-AP)

    IHC staining of mouse testis using 17857-1-AP (same clone as 17857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DDX10 Polyclonal antibody (17857-1-AP)

    IHC staining of human kidney using 17857-1-AP (same clone as 17857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DDX10 Polyclonal antibody, PBS Only

DDX10 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17857-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

DEAD box protein 10, EC:3.6.4.13, HRH J8, Probable ATP-dependent RNA helicase DDX10

Formulation:  PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17857-1-PBS targets DDX10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12317

Product name: Recombinant human DDX10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-431 aa of BC091521

Sequence: IGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETVSFHATDLQMLVLDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLARLSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLL

相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Calculated molecular weight 875 aa, 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC091521
Gene Symbol DDX10
Gene ID (NCBI) 1662
RRIDAB_2878454
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13206
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).

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