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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDRGK1 Polyclonal antibody (21445-1-AP)

    WB analysis using 21445-1-AP (same clone as 21445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21445-1-AP (DDRGK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DDRGK1 Polyclonal antibody (21445-1-AP)

    IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DDRGK1 Polyclonal antibody (21445-1-AP)

    IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using DDRGK1 Polyclonal antibody (21445-1-AP)

    IHC staining of human pancreas using 21445-1-AP (same clone as 21445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using DDRGK1 Polyclonal antibody (21445-1-AP)

    IHC staining of human pancreas using 21445-1-AP (same clone as 21445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDRGK1 Polyclonal antibody (21445-1-AP)

    IF Staining of HepG2 using 21445-1-AP (same clone as 21445-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDRGK1 antibody (21445-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DDRGK1 Polyclonal antibody, PBS Only

DDRGK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21445-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21445-1-PBS targets DDRGK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13742

Product name: Recombinant human DDRGK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-314 aa of BC000643

Sequence: MVAPVWYLVAAALLVGFILFLTRSRGRAASAGQEPLHNEELAGAGRVAQPGPLEPEEPRAGGRPRRRRDLGSRLQAQRRAQRVAWAEADENEEEAVILAQEEEGVEKPAETHLSGKIGAKKLRKLEEKQARKAQREAEEAEREERKRLESQREAEWKKEEERLRLEEEQKEEEERKAREEQAQREHEEYLKLKEAFVVEEEGVGETMTEEQSQSFLTEFINYIKQSKVVLLEDLASQVGLRTQDTINRIQDLLAEGTITGVIDDRGKFIYITPEELAAVANFIRQRGRVSIAELAQASNSLIAWGRESPAQAPA

相同性解析による交差性が予測される生物種
Full Name DDRGK domain containing 1
Calculated molecular weight 314 aa, 36 kDa
Observed molecular weight 36-46 kDa
GenBank accession numberBC000643
Gene Symbol DDRGK1
Gene ID (NCBI) 65992
RRIDAB_2827383
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HY6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.

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