Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, U-937 cells, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
13968-1-AP targets DDI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DDI1 fusion protein Ag4992 相同性解析による交差性が予測される生物種 |
| Full Name | DDI1, DNA-damage inducible 1, homolog 1 (S. cerevisiae) |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 47 kDa, 56 kDa |
| GenBank accession number | BC022017 |
| Gene Symbol | DDI1 |
| Gene ID (NCBI) | 414301 |
| RRID | AB_2089244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WTU0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ddi1(DNA-damage inducible 1)appears to release substrate from an ubiquitination complex, making the substrate available for deubiquitination to facilitate proteasomal degradation. Ddi1 could be a potential selective target for lung cancer treatment. Ddi1 runs as a doublet of 54/56 kDa on acrylamide gels besides the caculated MW 44 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDI1 antibody 13968-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Mol Histol Silibinin ameliorates cisplatin-induced acute kidney injury via activating Nfe2l1-mediated antioxidative response to suppress the ROS/MAPK signaling pathway. | ||
Int J Mol Sci The Effects of DDI1 on Inducing Differentiation in Ovine Preadipocytes via Oar-miR-432 |