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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDI1 Polyclonal antibody (13968-1-AP)

    WB analysis using 13968-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13968-1-AP (DDI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using DDI1 Polyclonal antibody (13968-1-AP)

    WB analysis of U-937 using 13968-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13968-1-AP (DDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DDI1 Polyclonal antibody (13968-1-AP)

    WB analysis of mouse testis using 13968-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13968-1-AP (DDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DDI1 Polyclonal antibody

DDI1 Polyclonal Antibody for WB, ELISA
Cat No. 13968-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

DDI1, Protein DDI1 homolog 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, U-937 cells, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13968-1-AP targets DDI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4992

Product name: Recombinant human DDI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-396 aa of BC022017

Sequence: MLITVYCVRRDLSEVTFSLQVSPDFELRNFKVLCEAESRVPVEEIQIIHMERLLIEDHCSLGSYGLKDGDIVVLLQKDNVGPRAPGRAPNQPRVDFSGIAVPGTSSSRPQHPGQQQQRTPAAQRSQGLASGEKVAGLQGLGSPALIRSMLLSNPHDLSLLKERNPPLAEALLSGSLETFSQVLMEQQREKALREQERLRLYTADPLDREAQAKIEEEIRQQNIEENMNIAIEEAPESFGQVTMLYINCKVNGHPLKAFVDSGAQMTIMSQACAERCNIMRLVDRRWAGVAKGVGTQRIIGRVHLAQIQIEGDFLQCSFSILEDQPMDMLLGLDMLRRHQCSIDLKKNVLVIGTTGTQTYFLPEGELPLCSRMVSGQDESSDKEITHSVMDSGRKEH

相同性解析による交差性が予測される生物種
Full Name DDI1, DNA-damage inducible 1, homolog 1 (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight 47 kDa, 56 kDa
GenBank accession numberBC022017
Gene Symbol DDI1
Gene ID (NCBI) 414301
RRIDAB_2089244
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WTU0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ddi1(DNA-damage inducible 1)appears to release substrate from an ubiquitination complex, making the substrate available for deubiquitination to facilitate proteasomal degradation. Ddi1 could be a potential selective target for lung cancer treatment. Ddi1 runs as a doublet of 54/56 kDa on acrylamide gels besides the caculated MW 44 kDa.

Protocols

Product Specific Protocols
WB protocol for DDI1 antibody 13968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Mol Histol

Silibinin ameliorates cisplatin-induced acute kidney injury via activating Nfe2l1-mediated antioxidative response to suppress the ROS/MAPK signaling pathway.

Authors - Fang Yang
View Article
sheepWB

Int J Mol Sci

The Effects of DDI1 on Inducing Differentiation in Ovine Preadipocytes via Oar-miR-432

Authors - Meilin Jin
View Article