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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDHD1 Polyclonal antibody

DDHD1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 26297-1-AP

Synonyms

DDHD domain containing 1, DDHD1, KIAA1705, PA PLA1, Phospholipase DDHD1

Validation Data Gallery

Applications:
  • WB analysis using 26297-1-AP

    WB analysis using 26297-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26297-1-AP (DDHD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 26297-1-AP

    IP experiment of mouse brain using 26297-1-AP

    IP result of anti-DDHD1 (IP:26297-1-AP, 4ug; Detection:26297-1-AP 1:800) with mouse brain tissue lysate 1680 ug.

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

26297-1-AP targets DDHD1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDHD1 fusion protein Ag23620 相同性解析による交差性が予測される生物種
Full Name DDHD domain containing 1
Observed molecular weight100-110 kDa
GenBank accession numberBC030703
Gene symbol DDHD1
Gene ID (NCBI) 80821
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDHD1, also known as KIAA1705, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD1 is highly expressed in the brain and testes. DDHD1 negatively controls the formation of a local phosphatidic acid-rich domain in recycling endosomes that serves as a membrane source for neurite outgrowth (PMID: 32850804).

Protocols

Product Specific Protocols
WB protocol for DDHD1 antibody 26297-1-APDownload protocol
IP protocol for DDHD1 antibody 26297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols