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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis using 66010-1-Ig (same clone as 66010-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis of A549 using 66010-1-Ig (same clone as 66010-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis of MCF-7 using 66010-1-Ig (same clone as 66010-1-PBS)

    N/A incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    WB analysis of MCF-7 using 66010-1-Ig (same clone as 66010-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human appendicitis using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human appendicitis using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human colon cancer using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DDB1 Monoclonal antibody (66010-1-Ig)

    IHC staining of human colon cancer using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DDB1 Monoclonal antibody (66010-1-Ig)

    IF Staining of MCF-7 using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunofluorescent analysis of fixed MCF-7 cells using 66010-1-Ig (DDB1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DDB1 Monoclonal antibody, PBS Only

DDB1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66010-1-PBS
Clone No.4F6C6

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

DDB p127 subunit, DDB1, DDBA, DNA damage binding protein 1, DNA damage binding protein a, HBV X associated protein 1, UV damaged DNA binding factor, UV DDB 1, UV DDB1, XAP 1, XAP1, XPCE, XPE, XPE BF, XPE binding factor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66010-1-PBS targets DDB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17903

Product name: Recombinant human DDB1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-400 aa of BC011686

Sequence: MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHA

相同性解析による交差性が予測される生物種
Full Name damage-specific DNA binding protein 1, 127kDa
Calculated molecular weight 1140 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC011686
Gene Symbol DDB1
Gene ID (NCBI) 1642
RRIDAB_10950859
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16531
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.