Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, fetal human brain tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84595-4-RR targets DCX in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4945 Product name: Recombinant human DCX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC027925 Sequence: MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM 相同性解析による交差性が予測される生物種 |
| Full Name | doublecortin |
| Calculated molecular weight | 402 aa, 45 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC027925 |
| Gene Symbol | DCX |
| Gene ID (NCBI) | 1641 |
| RRID | AB_3743594 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O43602 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DCX antibody 84595-4-RR | Download protocol |
| WB protocol for DCX antibody 84595-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |